Fig. 2: ANXA2 interacts with TRIM21 and counteracts on TRIM21-induced autophagy. | Cell Death & Disease

Fig. 2: ANXA2 interacts with TRIM21 and counteracts on TRIM21-induced autophagy.

From: TRIM21-regulated Annexin A2 plasma membrane trafficking facilitates osteosarcoma cell differentiation through the TFEB-mediated autophagy

Fig. 2

A U2-OS cells transfected with si-NC or si-TRIM21 were performed the IF assay, then the colocalization of TRIM21 and ANXA2 was observed using a confocal microscope (upper panel). The lower is the percentage of cells exhibiting colocalization of TRIM21 with ANXA2 of 80 cells. Scale bar: 10 μm. B U2-OS cells transfected with HA-vector or HA-TRIM21 were used to perform co-IP assay with HA antibody. The immune complexes of the HA antibody were analyzed by immunoblotting with the indicated antibodies. C U2-OS cells were used to perform co-IP assay with ANXA2 antibody. U2-OS cells transfected with si-ANXA2, si-NC (D), or EGFP-ANXA2, EGFP-V (E) in combination with CQ (5 μM, 2 h) treatment as indicated were performed western blotting assay. The corresponding quantitative analyses of LC3-II/β-tubulin or p62/β-tubulin ratios were shown in their lower panels, respectively (n = 3, SEM). F U2-OS cells stably expressing H125-TRIM21 or control H125-V were transfected with EGFP-ANXA2 or EGFP-vector and treated with TET (10 μg/ml, 12 h) to perform western blotting assay. LC3-II/β-tubulin was shown in its lower panel (n = 3, SEM). *p < 0.05, **p < 0.01, ***p < 0.001. ns: not significant. All data were representative of three independent experiments.

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