Fig. 2: The effects of CRC cell exosome on the expression of CRNDE-h in CD4+ T cells. | Cell Death & Disease

Fig. 2: The effects of CRC cell exosome on the expression of CRNDE-h in CD4+ T cells.

From: Tumor exosome promotes Th17 cell differentiation by transmitting the lncRNA CRNDE-h in colorectal cancer

Fig. 2

A The expressions of CRNDE-h in SW480-exosome (SW480-E), HT29-exosome (HT29-E), LOVO-exosome (LOVO-E), (LOVO-E), and normal colon epithelial cell-exosome (NCM460-E) were detected by qRT-PCR. B The SW480-E, HT29-E, LOVO-E, and NCM460-E (20 μg/mL) were co-cultured with naive CD4+ T cells isolated from the PMBCs of healthy subjects for 24 h. Then, the expression of CRNDE-h in T cells was detected by qRT-PCR. The “μg/mL” in this study means the protein concentration of exosomes. C CRC cells (SW480, HT29, and LOVO) were transfected with si-CRNDE-h (or si-control), and then used to isolate exosomes. Exosomes were co-cultured with naive CD4+ T cells isolated from the PMBCs of healthy subjects. Then, the expression of CRNDE-h in T cells was detected by qRT-PCR. E: exosome. **p < 0.01 vs. NCM460-E or si-control-E.

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