Fig. 2: MiR-126 promotes endothelial cell proliferation, migration, angiogenesis, and inhibits apoptosis. | Cell Death & Disease

Fig. 2: MiR-126 promotes endothelial cell proliferation, migration, angiogenesis, and inhibits apoptosis.

From: Down-regulation of long non-coding RNA HOTAIR promotes angiogenesis via regulating miR-126/SCEL pathways in burn wound healing

Fig. 2

a miR-126, HOTAIR, and SCEL levels in HUVECs exposed to heat stress were determined by qRT-PCR. U6 or GAPDH served as an internal control. b HUVECs were transfected with inhibitor NC or miR-126 inhibitor. miR-126, HOTAIR, and SCEL levels in HUVECs exposed to heat stress were determined by qRT-PCR. U6 or GAPDH served as an internal control. c Cell viability was monitored by the MTT assay. d Cell migration capacity was monitored by transwell migration assay. Data were representative images or were expressed as the mean ± SD of n = 3 experiments. e Cell apoptosis was determined by Annexin-V-FITC/PI staining followed by flow cytometry. f In vitro angiogenesis was quantified by tube formation assays. g Protein levels of SCEL, Col1a2, MMP9, and VEGFA were determined by western blotting. GAPDH served as loading control. Data were representative images or were expressed as the mean ± SD of n = 3 experiments. *P < 0.05 and **P < 0.01.

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