Fig. 6: SCEL is a direct target of miR-126. | Cell Death & Disease

Fig. 6: SCEL is a direct target of miR-126.

From: Down-regulation of long non-coding RNA HOTAIR promotes angiogenesis via regulating miR-126/SCEL pathways in burn wound healing

Fig. 6

a The predicted binding sites between miR-126 and SCEL 3′-UTR. A mutation was generated in 3′-UTR of SCEL in the complementary site for miR-126 binding. b Co-transfection of WT/MUT SCEL 3′-UTR and mimics NC/miR-126 mimics/inhibitor NC/miR-126 inhibitor in HEK293T cells. Firefly luciferase activity was examined by dual luciferase reporter assay. Renilla luciferase activity was used to normalize the activity of firefly luciferase. Data were representative images or were expressed as the mean ± SD of n = 3 experiments. c, d miR-126 and SCEL levels in HUVECs were determined by qRT-PCR. U6 or GAPDH served as an internal control. *P < 0.05, **P < 0.01.

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