Fig. 6: MiR-567 reverses trastuzumab resistance by inhibiting ATG5 and autophagy. | Cell Death & Disease

Fig. 6: MiR-567 reverses trastuzumab resistance by inhibiting ATG5 and autophagy.

From: RETRACTED ARTICLE: Exosome-transmitted miR-567 reverses trastuzumab resistance by inhibiting ATG5 in breast cancer

Fig. 6

a Western blotting showed that silence of ATG5 reversed the miR-567 inhibition-induced upregulation of LC3 and downregulation of p62 in SKBR-3-TR cells. b Knockdown of ATG5 abrogated the miR-567 inhibitor-induced LC3 puncta formation in SKBR-3-TR cells, **P < 0.01 compared with miR-567 inhibitor + si-NC group. c Western blotting showed that increased ATG5 reversed the miR-567 mimics-induced suppression of LC3 and increase of p62 in SKBR-3-TR cells. d Overexpression of ATG5 reversed the miR-567 mimics-induced LC3 puncta suppression in SKBR-3 cells, **P < 0.01 compared with miR-567 + p-NC group. e CCK8 assay showed that knockdown of ATG5 significantly reversed trastuzumab resistance, **P < 0.01. f Enhanced ATG5 level induced chemoresistance to trastuzumab of SKBR-3 and BT474 cells, *P < 0.05, **P < 0.01. g, h Knockdown of ATG5 reversed the miR-567 inhibitor-caused chemoresistance (g), whereas overexpression of ATG5 abrogated the miR-567 mimics-induced chemosensitivity (h).

Back to article page