Fig. 7: FXN deficiency impairs thermogenic program also in a brown adipocytes cell line. | Cell Death & Disease

Fig. 7: FXN deficiency impairs thermogenic program also in a brown adipocytes cell line.

From: Frataxin deficiency induces lipid accumulation and affects thermogenesis in brown adipose tissue

Fig. 7

a Representative immunofluorescence analysis of LDs after staining with the neutral lipid probe Nile Red in T37i brown adipocytes transfected with a pool of siRNAs targeting FXN mRNA (FXN) or with a pool of Scr siRNAs (Scr) (n = 4). b, c Representative immunoblots of FXN, total and phospho-active HSL (p-HSL660) b, PPARγ, TOM20, VDAC, and UCP1 c (n = 4). d RT-qPCR analysis of genes implicated in thermogenesis (n = 4; **p < 0.01,***p < 0.001 vs. Scr; °°p < 0.01, °°°p < 0.001 vs. FXN-; ##p < 0.01, ###p < 0.001 vs. Scr + IBMX). e Cellular oxygen consumption determined through a polarographic method (n = 4, **p < 0.01 vs. Scr, °p < 0.05 vs. FXN−; ###p < 0.001 vs. Scr + IBMX).

Back to article page