Fig. 8: KIKO mice show affected adipogenic potential and increased ferroptosis susceptibility.
From: Frataxin deficiency induces lipid accumulation and affects thermogenesis in brown adipose tissue
a Representative images of stromal vascular cells (SVCs) obtained from mouse adipose depots of WT or KIKO mice, differentiated in adipocytes and stained with Oil Red O to detect LD-containing mature adipocytes (n = 3). b RT-qPCR analysis of genes implicated in adipogenesis, thermogenesis, and iron metabolism in SVCs differentiated in adipocytes (n = 6; ***p < 0.001 vs. WT). c–e Analysis of cell death through trypan blue staining c, representative immunoblots of GPX4 d, and lipid peroxidation (left and right panels) through C11-Bodipy staining e in SVCs obtained from mouse depots of WT and KIKO mice and treated with 1 mM BSO for 72 h (n = 3, *p < 0.05, **p < 0.01 vs. WT; °p < 0.05, °°p < 0.01 vs. BSO-treated WT). f Representative images of mouse embryonic fibroblasts (MEFs) obtained from WT or KIKO mouse embryos, differentiated in adipocytes and stained with Oil Red O to detect LD-containing mature adipocytes (n = 3). g RT-qPCR analysis of genes implicated in adipogenesis, thermogenesis, ferroptosis, and iron metabolism in MEFs differentiated in adipocytes (n = 6; ***p < 0.001 vs. WT).
