Fig. 4: TUG1 regulates EMT in CRC cells. | Cell Death & Disease

Fig. 4: TUG1 regulates EMT in CRC cells.

From: The long noncoding RNA TUG1 is required for TGF-β/TWIST1/EMT-mediated metastasis in colorectal cancer cells

Fig. 4

a The expression levels of vimentin and E-cadherin in LoVo, HT-29, and HCT116 cells were detected by western blot following treatment with 3 ng/ml TGF-β or 2 μm LY 364947 for 48 h. *P < 0.05, **P < 0.01, and ***P < 0.001, versus control. b, c Expression of vimentin and E-cadherin in LoVo, HT-29, and HCT116 cells transfected with NC-siRNA or TUG1 siRNA was analyzed by western blot and immunofluorescence, *P < 0.05 and **P < 0.01, versus NC-siRNA. Scale bar, 100 μm. d LoVo, HT-29, and HCT116 cells transfected with TUG1 siRNA were treated with 3 ng/ml TGF-β and expression of vimentin and E-cadherin was evaluated by western blot.

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