Fig. 4: The proliferation and cell death effects of TNF-α are mediated by TNFR1. | Cell Death & Disease

Fig. 4: The proliferation and cell death effects of TNF-α are mediated by TNFR1.

From: The concentration of tumor necrosis factor-α determines its protective or damaging effect on liver injury by regulating Yap activity

Fig. 4

a Western blot analysis of TNFR1 in BRL cells transfected with control-siRNA or TNFR1-siRNA. b BRL cells were transfected with control-siRNA or transfected with TNFR1-siRNA for 12 h, and then treated with different concentrations of TNF-α (0, 10, or 500 ng/mL) for 6 h. Cell viability was determined using CCK-8 counts (n = 6 for each group). c, d Apoptotic cells were assayed using an Annexin V-FITC/PI double staining flow cytometry assay (n = 3 for each group). The cells Data are expressed as the mean ± SD. *p < 0.05. Data are representative of at least three independent experiments.

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