Fig. 3: PABPC1 binds to BDNF-AS and stabilizes BDNF-AS. | Cell Death & Disease

Fig. 3: PABPC1 binds to BDNF-AS and stabilizes BDNF-AS.

From: PABPC1-induced stabilization of BDNF-AS inhibits malignant progression of glioblastoma cells through STAU1-mediated decay

Fig. 3

a Expression of BDNF-AS after PABPC1 overexpression or knockdown (n = 3, each group). **P < 0.01 vs. PABPC1(+)NC group. ##P < 0.01 vs. PABPC1(−)NC group. b Identification of the newly synthesized RNA enrichment of BDNF-AS via RNA capture assay and qRT-PCR. c Half-life of BDNF-AS measured by qRT-PCR and normalized to the level of GAPDH. d RIP was performed in U87 and U251 cells and followed by qRT-PCR to detect BDNF-AS associated with PABPC1. **P < 0.01 vs. anti-IgG group. e Western blot analysis following RNA pull-down assays performed using U87 and U251 cellular exacts. f Effect of PABPC1 and BDNF-AS on cell proliferation, g apoptosis, h migration and invasion of glioblastoma cells. Scale bars represent 40 μm. (n = 5, each group). **P < 0.01 vs. control group; ##P < 0.01 vs. PABPC1(+) + BDNF-AS(+) group. ▵▵P < 0.01 vs. PABPC1(+) + BDNF-AS(−) group.

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