Fig. 8: RAX2 increased the expression of DLG5 by binding to the promoter of DLG5. | Cell Death & Disease

Fig. 8: RAX2 increased the expression of DLG5 by binding to the promoter of DLG5.

From: PABPC1-induced stabilization of BDNF-AS inhibits malignant progression of glioblastoma cells through STAU1-mediated decay

Fig. 8

a The DLG5 mRNA expression after RAX2 knockdown was detected via qRT-PCR. b Western blot assay was used to detect the protein expression of DLG5 after RAX2 knockdown (n = 3, each group). **P < 0.01 vs. RAX2(−)NC group. c RAX2 affect on DLG5 promoter activity. d RAX2 bound to the promoter of DLG5 in U87 and U251 cells. Schematic representation of the human DLG5 promoter region 4000 bp upstream of the transcription start site (TSS), which was designated as +1. Putative RAX2-binding site was indicated. PCR was conducted with the resulting precipitated DNA. e Western blot assay of the p-LATS1/LATS and p-YAP/YAP expression regulated by DLG5. **P < 0.01 vs. DLG5(+)NC group; ##P < 0.01 vs. DLG5(−)NC group.

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