Fig. 2: miR-210-5p promotes tumor invasion and migration in OS cells. | Cell Death & Disease

Fig. 2: miR-210-5p promotes tumor invasion and migration in OS cells.

From: miR-210-5p promotes epithelial–mesenchymal transition by inhibiting PIK3R5 thereby activating oncogenic autophagy in osteosarcoma cells

Fig. 2

a A gene set enrichment analysis (GSEA) was used to compare the high miR-210-5p group (red) with the low miR-210-5p group (blue) among the OS cohorts in the TCGA data set. Higher miR-210-5p expression was correlated with the EMT process. b Immunohistochemical staining of vimentin in OS tissues. Scale bar = 100 μm. c Western blot analysis of EMT and invasion-related protein levels in transfected HOS and MG63 cells. Protein levels on western blot were quantified by densitometry of N-cadherin, E-cadherin, vimentin, and MMP2, normalized to GAPDH. d, e Effects of miR-210-5p on invasion in vitro using the transwell invasion assay. Quantification of the transwell invasion assay is shown. Scale bar = 200 μm. f, g Wound-healing assay was performed using HOS and MG63 cells, and cell migration was measured 24 h after scratching. Scale bar = 250 μm. h, i Representative images of 3D spheroid BME cell-invasion assay in transfected HOS and MG63 cells. Quantification of 3D spheroid BME cell-invasion assay is shown. Scale bar = 250 μm.

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