Fig. 3: miR-210-5p promotes autophagy in OS cells. | Cell Death & Disease

Fig. 3: miR-210-5p promotes autophagy in OS cells.

From: miR-210-5p promotes epithelial–mesenchymal transition by inhibiting PIK3R5 thereby activating oncogenic autophagy in osteosarcoma cells

Fig. 3

a Effects of miR-210-5p on the regulation of autophagy and autophagosome formation analyzed by GSEA. b Immunohistochemical staining of p62 in OS specimens. Scale bar = 100 μm. c TEM was used to detect the autophagic microstructure of transfected OS cells. Red arrows indicate a cellular autophagosome with a double-layer structure or an autolysosome generated by the fusion of an autophagosome and lysosome. d, e OS cells transfected with a GFP-mRFP-LC3 lentivirus were cultured, and yellow and red puncta were observed and counted under confocal microscopy. Scale bar = 20 μm. f Western blot analysis of autophagy-related protein levels including Beclin1, p62, and ATG5 in transfected OS cells. g Western blot analysis of LC3-II and p62 in transfected OS cells treated with the autophagy flux inhibitor chloroquine (CQ, 10 μM for 6 h).

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