Fig. 5: miR-210-5p promotes tumor invasion and migration by suppressing PIK3R5 in OS cells. | Cell Death & Disease

Fig. 5: miR-210-5p promotes tumor invasion and migration by suppressing PIK3R5 in OS cells.

From: miR-210-5p promotes epithelial–mesenchymal transition by inhibiting PIK3R5 thereby activating oncogenic autophagy in osteosarcoma cells

Fig. 5

a–g A series of gain- and loss-of-function experiments including western blot (a), transwell invasion assay (b, c), wound-healing assay (d, e), and 3D spheroid BME cell-invasion assay (f, g) were carried out to confirm the functional relationship between miR-210-5p and PIK3R5. a Western blot analysis was conducted to evaluate the EMT and invasion-related protein levels. Rescue experiments for miR-210-5p overexpression were conducted via the ectopic expression of PIK3R5 in HOS cells. Rescue experiments for miR-210-5p inhibition were conducted by downregulating PIK3R5 in MG63. b–g Rescue experiments were also conducted using the transwell invasion assay (scale bar = 200 μm), wound-healing assay (scale bar = 250 μm), and 3D spheroid BME cell-invasion assay (scale bar = 250 μm).

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