Fig. 6: PIK3R5 reversed the effects of miR-210-5p on autophagy in OS cells. | Cell Death & Disease

Fig. 6: PIK3R5 reversed the effects of miR-210-5p on autophagy in OS cells.

From: miR-210-5p promotes epithelial–mesenchymal transition by inhibiting PIK3R5 thereby activating oncogenic autophagy in osteosarcoma cells

Fig. 6

a–c HOS and MG63 cells transfected with GFP-mRFP-LC3 lentivirus were observed, and the cellular puncta were counted under confocal microscopy. Rescue experiments for miR-210-5p overexpression were conducted via the ectopic expression of PIK3R5 in HOS cells. Rescue experiments for miR-210-5p inhibition were conducted by downregulating PIK3R5 in MG63 cells. Scale bar = 20 μm. d Western blot analysis was used to confirm the results obtained from confocal images.

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