Fig. 6: Pten deletion and Pten inhibition regulate lineage choice and differentiation in mouse EBs.
From: Pten-mediated Gsk3β modulates the naïve pluripotency maintenance in embryonic stem cells
a RT-PCR analysis of EBs derived from WT and Pten−/− ESCs showing the expression of lineage-specific markers. b Scatter plot of transcript expression in WT and Pten−/− EBs. Expression values are shown on a log10 scale. Yellow dots indicate upregulated genes in Pten−/− EBs, and blue dots indicate downregulated genes. Probability ≥ 0.8 and abs (log2(Y/X)) ≥ 1. c Heat map of FPKM values of pluripotency, ectoderm, endoderm, and mesoderm markers in WT and Pten−/− EBs. The heat map was normalized with sigma-normalization per row. See also Table S4. d The expression of lineage-specific markers was assessed by qRT-PCR in WT and Pten−/− EBs. Error bars indicate mean ± SD (n = 3). e The expression of lineage-specific markers was assessed by qRT-PCR in WT EBs treated with DMSO or SF1670. Error bars indicate mean ± SD (n = 3). f Expression of ectoderm markers Pax6 and Sox1 in WT and Pten−/− EBs at different days. Error bars indicate mean ± SD (n = 3).
