Fig. 2: RUNX1-IT1 restrains HCC proliferation, cell cycle and facilitates cell apoptosis in vitro. | Cell Death & Disease

Fig. 2: RUNX1-IT1 restrains HCC proliferation, cell cycle and facilitates cell apoptosis in vitro.

From: LncRNA RUNX1-IT1 which is downregulated by hypoxia-driven histone deacetylase 3 represses proliferation and cancer stem-like properties in hepatocellular carcinoma cells

Fig. 2

a The expression level of RUNX1-IT1 in MHCC-97H cells was enhanced by pcDNA/RUNX1-IT1, and RUNX1-IT1 expression in HepG2 cells was reduced by sh-RUNX1-IT1 (shRNA#1, shRNA#2). n = three independent experiments, ***P < 0.001 by Student’s t test versus vector, or **P < 0.01 by ANOVA versus shVector. pcDNA/RUNX1-IT1 repressed whereas RUNX1-IT1 shRNA promoted cell viability b, cell proliferation c, colony formation d and cell cycle progression e. n = three independent experiments, *P < 0.05, **P < 0.01 or ***P < 0.001 by Student’s t test versus Vector or ANOVA versus shVector. f pcDNA/RUNX1-IT1 accelerated, whereas RUNXI-IT1 shRNA induced an inhibition of HCC cells apoptosis. n = three independent experiments, **P < 0.01 by Student’s t test versus Vector or ANOVA versus shVector. Magnification of Edu is ×200, and scale bars = 50 μm. The scale bars of colony formation = 1 cm.

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