Fig. 4: RUNX1-IT1 inhibits the tumorgenicity and metastasis of MHCC-97H cells along with reversing the mesenchymal and stem-like phenotypes.

a Representative images of subcutaneous xenografts in nude mice implanted with MHCC-97H cells with Vector or overexpression RUNX1-IT1. (n = 6 per group). b, c Xenografts weight (mg) and tumour sizes were monitored and undergone quantification analysis. n = 6, **P < 0.01 by Student’s t test for tumour weight; **P < 0.01 by repeated-measures ANOVA for tumour sizes. d Immunohistochemistry staining and the semi-quantification analysis of Ki-67, E-cadherin, Vimentin, CD44, Nanog and Sox2 in xenograft tissues from different groups. Magnification is ×400, the scale bar represents 20 μm. n = 6, *P < 0.05 or **P < 0.01 by Student’s t test. e Representative images of orthotopic liver xenografts in nude mice implanted with MHCC-97H cells with Vector or overexpression RUNX1-IT1, representative hematoxylin and eosin–stained sections, and quantification analysis of tumour nodules in livers were displayed. Magnification is ×100, the scale bar represents 50 μm. n = 6, **P < 0.01 by Student’s t test. f Representative hematoxylin and eosin–stained sections, and quantification analysis of metastasis nodules in lungs were shown. Magnification is ×100, the scale bar represents 50 μm. n = 6, **P < 0.01 by Student’s t test.