Fig. 4: Antibody-mediated Nogo-A blockade decreases the number of cells expressing TNFα without affecting the number of Iba1-positive monocytes. | Cell Death & Disease

Fig. 4: Antibody-mediated Nogo-A blockade decreases the number of cells expressing TNFα without affecting the number of Iba1-positive monocytes.

From: Nogo-A-targeting antibody promotes visual recovery and inhibits neuroinflammation after retinal injury

Fig. 4

Cells expressing TNFα, Iba1, and CD68 were labeled on cryosections by immunofluorescence in 4 mouse retinae, 24 h after control IgG or 11C7 injection. a Retinal sections showed many TNFα-containing cells located in the inner retina, between the outer plexiform layer and the ganglion cell layer 1 day after control IgG injection. A majority of cells was positive for Iba1 and exhibited vesicular staining of the lysosomal protein CD68, indicating their active state. b The injection of 11C7 strongly attenuated TNFα and CD68 expression and to a lower extent the intensity of Iba1 in macrophages/microglia. c Quantification of the number of cells expressing TNFα and Iba1 suggesting potent and specific effects of 11C7 on TNFα cytokine reduction. Six sections were examined for each retina. Statistics: one-way ANOVA, Dunnett’s post hoc test, **P < 0.01; ****P < 0.0001. Scale bars = 50 μm.

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