Fig. 2: Metformin reversed the alectinib resistance induced by HGF/MET in vitro.
From: Metformin reduces HGF-induced resistance to alectinib via the inhibition of Gab1
a The alectinib IC50 values of H3122-AR3 cells with or without JNJ-38877605 (10 nmol/L) or metformin (5 mmol/L) treatment. **p < 0.001. b Metformin reversed alectinib resistance induced by exogenous or endogenous HGF in H3122 cells. The alectinib IC50 values of H3122 cells, H3122/Vec cells, and H3122/HGF cells with or without HGF (50 ng/mL) and metformin (5 mmol/L) treatment were detected by MTT assay. The data are presented as the mean ± SD from three independent experiments. **p < 0.001. c Metformin (5 mmol/L) and alectinib (50 nmol/L) synergistically inhibited the proliferation of H3122/Vec cells, H3122/HGF cells and H3122-AR3 cells, as determined by a Ki67-incorporation assay. The data are presented as the mean ± SD from three independent experiments. *p < 0.05; **p < 0.001. d Metformin (5 mmol/L) and alectinib (50 nmol/L) synergistically inhibited the clone-forming ability of H3122/Vec cells and H3122/HGF cells. *p < 0.05; **p < 0.001. Ale alectinib, METi MET selective inhibitor JNJ-38877605, Metf metformin, Vec negative control vector.
