Fig. 2: Manual scoring confirms association of low Bak, Bax and Smac protein expression with improved PFS.

a Correlation between automated and manual H scores. Manual scores were obtained from two independent pathologists blinded to patient follow-up. Correlation was analysed using Spearman’s rank correlation coefficient. b Median H scores were used as cutoff to separate the patients with high (red line) and low (blue line) expression of each protein. Log-rank test was used to compare the Kaplan–Meier curves for progression-free survival from the date of sample procurement.