Fig. 2: APAP induces Ptgs2 expression, lipid peroxidation, and GSH depletion. | Cell Death & Disease

Fig. 2: APAP induces Ptgs2 expression, lipid peroxidation, and GSH depletion.

From: Ferroptosis driven by radical oxidation of n-6 polyunsaturated fatty acids mediates acetaminophen-induced acute liver failure

Fig. 2

Liver samples were obtained from mice injected with vehicle or APAP (200 mg/kg) 3 h after injection. Mice were treated with Fer-1 (10 mg/kg) or vehicle 1 h prior to injection. a Hepatic PTGS2 mRNA expression was assessed (n = 4–6 for each). b Liver sections were analyzed by immunohistochemical staining for 4-HNE (n = 4–6 for each). c Hepatic MDA levels were assessed by MDA-TBARS assay (n = 5 for each). d Liver samples were analyzed by western blotting for xCT. β-actin served as a loading control. e Liver samples were analyzed by western blotting for GPX4. β-actin served as a loading control. f Hepatic levels of total GSH (GSH + 2GSSG) and reductant GSH were assessed. GSH was calculated as total GSH –2GSSG (n = 4–6 for each). Statistical significance was calculated using the Mann–Whitney test with the Bonferroni correction a–e. Data are expressed as dot plots and/or means ± SEM. *p < 0.05, **p < 0.01.

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