Fig. 3: Fer-1 has no effects on other types of cell death or inflammatory responses. | Cell Death & Disease

Fig. 3: Fer-1 has no effects on other types of cell death or inflammatory responses.

From: Ferroptosis driven by radical oxidation of n-6 polyunsaturated fatty acids mediates acetaminophen-induced acute liver failure

Fig. 3

Liver samples were obtained from mice injected with vehicle or APAP (200 mg/kg) 3 h after injection. Mice were treated with Fer-1 (10 mg/kg) or vehicle 1 h prior to injection. a, b Liver samples were analyzed by western blotting for caspase-3, cleaved caspase-3, and RIP3K. DEX-treated thymus and L929 cells were used as positive controls for cleaved caspase-3 and RIP3K, respectively. β-actin served as a loading control. c Liver sections were analyzed by immunohistochemical staining for CD45 (n = 4–6 for each). d Hepatic mRNA expression of inflammatory cell markers (Ly6G and EMR) and cytokines (IL-1β, IL-6, TNF-α, and CCL2) was assessed (n = 4–6 for each). Statistical significance was calculated using the Mann–Whitney test with the Bonferroni correction a–d. Data are expressed as dot plots and/or means ± SEM.

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