Fig. 5: Arachidonic acid-derived lipid peroxidation is involved in APAP-induced hepatotoxicity. | Cell Death & Disease

Fig. 5: Arachidonic acid-derived lipid peroxidation is involved in APAP-induced hepatotoxicity.

From: Ferroptosis driven by radical oxidation of n-6 polyunsaturated fatty acids mediates acetaminophen-induced acute liver failure

Fig. 5

Liver samples were obtained from mice injected with vehicle or APAP (200 mg/kg) 3 h after injection. Mice were treated with Fer-1 (10 mg/kg) or vehicle 1 h prior to injection. a–d Liver samples were analyzed by LC-MS to identify lipid mediators and volcano plots are shown. a APAP vs. vehicle in n-6 fatty acid, b APAP + Fer-1 vs. vehicle in n-6 fatty acid, c APAP vs. vehicle in n-3 fatty acid, d APAP + Fer-1 vs. vehicle in n-3 fatty acid. e–h Relative expression levels of PGD2, LTC4, 12-HETE, 20-HETE, and 8-iso-PGF2α are shown. The data are expressed as relative expression compared with a reference standard (n = 3 for each). Statistical significance was calculated using Student’s t test e–h. Data are expressed as dot plots and/or means ± SEM. *p < 0.05, **p < 0.01.

Back to article page