Fig. 6: ACSL4 deletion attenuates APAP-induced hepatotoxicity and lipid peroxidation. | Cell Death & Disease

Fig. 6: ACSL4 deletion attenuates APAP-induced hepatotoxicity and lipid peroxidation.

From: Ferroptosis driven by radical oxidation of n-6 polyunsaturated fatty acids mediates acetaminophen-induced acute liver failure

Fig. 6

a Liver and serum samples were obtained from ACSL4+/Y and ACSL4–/Y mice injected with vehicle or APAP (200 mg/kg) 3 h after injection. Serum levels of AST and ALT were assessed (n = 3–5 for each). b–e The hydrodynamics-based transfection method of CRISPR/Cas9 genome editing was used to create hepatic ACSL4-deleted mice. Liver and serum samples were obtained from mice injected with vehicle or APAP (200 mg/kg) 3 h after injection. px330-ACSL4-sgRNA or GFP-sgRNA was injected by the hydrodynamics-based method 7 days prior to APAP injection. b Hepatic ACSL4 expression. c Serum AST and ALT levels (n = 3–5 for each). d HE staining. e Histopathological score. f Hepatic PTGS2 mRNA expression. g 4-HNE immunostaining. h Hepatic MDA levels. i Hepatic total GSH levels. j Hepatic CYP2E1 mRNA expression. Statistical significance was calculated using the Mann–Whitney test with the Bonferroni correction a–e. Data are expressed as dot plots and/or means ± SEM. *p < 0.05, **p < 0.01 (n = 6–8 for each).

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