Fig. 8: α-Tocopherol inhibits APAP-induced hepatotoxicity and lipid peroxidation.

Liver and serum samples were obtained from mice injected with vehicle or APAP (200 mg/kg) 3 h after injection. Mice were treated with α-tocopherol (α-Toc, 100 mg/kg/day) or vehicle 1 h prior to injection. a Serum AST and ALT levels. b, c HE staining and histopathology score. d Hepatic CYP2E1 mRNA expression. e 4-HNE immunostaining. f Hepatic MDA levels. g Hepatic total GSH levels. Statistical significance was calculated using Mann–Whitney test a–g with the Bonferroni correction. Data are expressed as dot plots and/or means ± SEM (n = 3–6 for each). *p < 0.05, **p < 0.01.