Fig. 1: circRNF20 is upregulated in the BC tissue and cells. | Cell Death & Disease

Fig. 1: circRNF20 is upregulated in the BC tissue and cells.

From: Circular RNA circRNF20 promotes breast cancer tumorigenesis and Warburg effect through miR-487a/HIF-1α/HK2

Fig. 1

a Heat map showed the upregulated circRNAs and downregulated circRNAs. b Sanger sequencing illustrated the joint site of circRNF20 (3’-end of exon 5 and 5’-end of exon 3). c Schematic diagram illustrated the formation of circRNF20 originated from RNF20 pre-mRNA (exon 3, 4, 5). d RT-PCR revealed the expression of circRNF20 in the BC cell lines. e RNA stability assay found the expression of circRNF20 or linear mRNA transcript in MDA-MB-231 cells under treatment with transcription inhibitor (actinomycin D). f RT-PCR showed the expression of circRNF20 and RNF20 mRNA in MDA-MB-231 cells administered with RNase R or Mock control. g RT-PCR showed the circRNF20 level in the BC tissue. h Prognosis analysis calculated by Kaplan-Meier and log-rank test unveiled the survival rate of BC patients with higher or lower circRNF20 expression. **p < 0.01 vs. control.

Back to article page