Fig. 2: circRNF20 promotes the progress and glycolysis of BC. | Cell Death & Disease

Fig. 2: circRNF20 promotes the progress and glycolysis of BC.

From: Circular RNA circRNF20 promotes breast cancer tumorigenesis and Warburg effect through miR-487a/HIF-1α/HK2

Fig. 2

a Stable oligonucleotides transfections were constructed, including circRNF20 knockdown (sh-circRNF20) in MDA-MB-231 cells and circRNF20 overexpression (circRNF20 OE) in MCF-7 cells. b CCK-8 proliferative assay showed the proliferation with the transfection of circRNF20 knockdown and circRNF20 overexpression. c Flow cytometry apoptosis assay indicated the apoptotic rate of MDA-MB-231 and MCF-7 cells. d Relative glucose uptake was detected in BC cells. e Relative lactate production was detected in BC cells. f Relative ATP level was detected in BC cells. g, h In vivo mice heterograft assay unveiled the tumor growth in mice injection using MDA-MB-231 cells transfected with circRNF20 knockdown. **p < 0.01 vs. control. *p < 0.05 vs. control.

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