Fig. 1: Differential expression of miRNAs during C2C12 myoblast differentiation. | Cell Death & Disease

Fig. 1: Differential expression of miRNAs during C2C12 myoblast differentiation.

From: Chromatin accessibility is associated with the changed expression of miRNAs that target members of the Hippo pathway during myoblast differentiation

Fig. 1

a Schematic for stimulating the differentiation of C2C12 myoblasts into myotubes and morphological images of C2C12 myoblasts cultured in GM or in DM for 0, 1, 2, and 4 days (×100). b Expression of MyoD and MyoG during cell differentiation as determined by Q-PCR. The expression fold change is relative to day 0 in GM culture. c Hierarchical clustering of differentially expressed miRNAs on days 0, 1, 2, and 4 during C2C12 differentiation. d Expression of miRNAs on days 0, 1, 2, and 4 of cell differentiation via stem-loop Q-PCR. The expression fold change is relative to day 0 in GM culture. e Correlation analysis of stem-loop Q-PCR and miRNA sequencing data. The x-axis and y-axis show the values obtained by Q-PCR and RNA-seq, respectively. GM, growth medium; DM, differentiation medium. The error bars indicate the standard errors of the mean from three independent experiments. *p < 0.05; **p < 0.01.

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