Fig. 3: KEGG pathway annotation and RNA-seq dataset analysis.

a KEGG pathways most significantly enriched in the top 20 differentially expressed miRNA targets ranked by the enrichment score (−log10(p value)). b Heat map and clustering patterns of miRNA targets in the Hippo signaling pathway during C2C12 myoblast differentiation as determined by rRNA-depleted RNA-seq data. c Expression of genes as quantified by Q-PCR. The expression fold change is relative to day 0 in GM culture. d Scatter plot showing the two general methodologies. The x-axis shows the values obtained by Q-PCR, and the y-axis shows the values obtained by RNA-seq. *p < 0.05; **p < 0.01.