Fig. 1: Apatinib inhibits the growth and promotes the apoptosis of GC cells in vitro and in vivo. | Cell Death & Disease

Fig. 1: Apatinib inhibits the growth and promotes the apoptosis of GC cells in vitro and in vivo.

From: Silencing of circRACGAP1 sensitizes gastric cancer cells to apatinib via modulating autophagy by targeting miR-3657 and ATG7

Fig. 1

a, b Colony-formation assay of BGC-823 cells and HGC-27 cells after apatinib treatment for 24 h. Quantification of numbers of colonies in BGC-823 cells and HGC-27 cells; each colony containing >50 cells was counted. c, d BGC-823 cells and HGC-27 cells were incubated with apatinib at various concentrations for 48 h. Then, apoptosis induced by apatinib was detected by Annexin V–FITC/PI staining and analyzed using flow cytometry. e–g Effects of apatinib on the growth of GC in vivo. Statistical analyses of tumor volumes (e) and body weights (f) in the different groups. Photograph of tumors, hematoxylin–eosin (HE) staining and immunohistochemical staining of tumor tissues (g) in mice treated with control vehicle or apatinib (n = 6 per subgroup). Bar scale, 100 μm. *P < 0.05; **P < 0.01; ***P < 0.001.

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