Fig. 2: Apatinib promotes GC autophagy activation in vitro and in vivo. | Cell Death & Disease

Fig. 2: Apatinib promotes GC autophagy activation in vitro and in vivo.

From: Silencing of circRACGAP1 sensitizes gastric cancer cells to apatinib via modulating autophagy by targeting miR-3657 and ATG7

Fig. 2

a–c qRT-PCR was performed in vivo (a) and in BGC-823 cells (b) and HGC-27 cells (c) cultured with apatinib. *P < 0.05; ***P < 0.001. d, e Western blotting was performed on autophagy proteins expressed in BGC-823 cells (d) and HGC-27 cells (e) co-cultured with apatinib at various concentrations for 48 h. f, g BGC-823 cells (f) and HGC-27 cells (g) were transfected with mRFP-GFP-LC3 plasmid, followed by incubation with apatinib. Confocal microscopy analysis is shown. Bar scale, 10 μm. h, i The numbers of GFP+ or RFP+ dots per cell are presented as the means ± SD of three independent experiments. **P < 0.01; ***P < 0.001 compared with the corresponding control group. #P < 0.05 compared with the GFP group treated with apatinib. j Immunohistochemical staining of LC3-II, SQSTM1, and ATG7 in the tumor xenograft tissues from the apatinib and control groups. Bar scale, 100 μm. k Autophagosomes were observed by TEM in tumor xenografts from the apatinib group. Bar scale, 500 nm (right, enlarged).

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