Fig. 3: CZ enhances MSCs immunosuppressive function in vitro. | Cell Death & Disease

Fig. 3: CZ enhances MSCs immunosuppressive function in vitro.

From: Chlorzoxazone, a small molecule drug, augments immunosuppressive capacity of mesenchymal stem cells via modulation of FOXO3 phosphorylation

Fig. 3

a, b Representative flow cytometry analysis of T-cell activation marker CD69 and CD25 (left) and statistical histogram of CD69 and CD25 expression on T cells (right) in different culturing groups. c Histogram of generations of T cells in different culturing groups measured by the CFSE assay in 3 days. d MTS assay of T cells proliferation in different culture groups for 4 days. e MTS assay of T cells co-cultured with MSCs treated by different concentrations of CZ for 4 days. f Histogram of quantitative RT-PCR of RNA expression (up) and western blotting of protein expression (down) of IDO in MSCs treated by different CZ concentrations. g Histogram of quantitative RT-PCR of gene expression of main cytokines and chemokines altered by different CZ concentrations in MSCs. *P < 0.05, **P < 0.01, ***P < 0.001.

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