Fig. 4: FOXO3 phosphorylation regulated by CZ. | Cell Death & Disease

Fig. 4: FOXO3 phosphorylation regulated by CZ.

From: Chlorzoxazone, a small molecule drug, augments immunosuppressive capacity of mesenchymal stem cells via modulation of FOXO3 phosphorylation

Fig. 4

a Western blotting of p-FOXO3 and total FOXO3 influenced by CZ dose gradation. One gel was run and two blots were made in this image. One blot for total protein and the other for phosphorylated protein. The same loading control of GAPDH was used as Fig. 3f. b Western blotting of p-FOXO3, total FOXO3, p-AKT, and p-ERK influenced by CZ, AKT, and/or ERK inhibitors. Two gels were run and two blots were made in this image. One blot for total protein and the other for phosphorylated protein. c Western blotting of p-FOXO3, total FOXO3, and p-AKT influenced by CZ and/or AKT activators. One gel was run and two blots were made in this image. One blot for total protein and the other for phosphorylated protein. All protein bands were visualized by chemiluminescent ECL reagent without antibody stripping procedure. d Immunofluorescence of total FOXO3 cellular location influenced by CZ and/or different kinase inhibitors and activators. Scale bars: 100 μm.

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