Fig. 2: Hyperglycemic microenvironment increases cell death and MMP9 activity in hCSCs. | Cell Death & Disease

Fig. 2: Hyperglycemic microenvironment increases cell death and MMP9 activity in hCSCs.

From: MMP9 mediates acute hyperglycemia-induced human cardiac stem cell death by upregulating apoptosis and pyroptosis in vitro

Fig. 2

The hCSCs were treated with normal (NG) or high (HG) dose of glucose for 24 h and subsequently used for analyses of cell death and MMP9 activity. a Representative TUNEL assay for apoptosis in hCSCs. TNFα + cyclohexamide treatment was a positive control for TUNEL. b Flow cytometry assay for Annexin V/DAPI, a marker of cell death, in HG or NG medium. c In-gel-gelatin zymography showing increased activity of MMP9 in hyperglycemic hCSCs. Values are expressed as mean ± SEM. Each dot represents one sample. Student t-test was used. P < 0.05 is considered statistically significant.

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