Fig. 5: TNFAIP8 inactivates AKT/mTOR and induces autophagy. | Cell Death & Disease

Fig. 5: TNFAIP8 inactivates AKT/mTOR and induces autophagy.

From: TNFAIP8 regulates autophagy, cell steatosis, and promotes hepatocellular carcinoma cell proliferation

Fig. 5

a Cell lysates from HepG2 and SK-Hep1 cells transfected with EV and TNFAIP8-Myc plasmids (left and middle panels) and EV or TNFAIP8-Myc-stable-expressing HepG2 cells (right panel) were WB with indicated antibodies. Indicated protein levels were quantified using ImageJ software (https://imagej.nih.gov/ij/). b HCC cells were treated with TNFα (10 to 50 ng/ml) for 30 h and lysates were WB with indicated antibodies. c, d HepG2 and SK-Hep1 cells were pretreated with 3-MA (2 mM) or chloroquine (10 µM) for 8 h and cells were transfected with EV or TNFAIP8-Myc plasmid in the presence of the indicated autophagy inhibitor for 40 h, and cell survival was measured by MTT assay. e, f HepG2 and SK-Hep1 cells were pretreated with 3-MA (2 mM) for 8 h, the cells were transfected with EV or TNFAIP8-Myc plasmid in the presence of autophagy inhibitor for 24 h and then treated with sorafenib (5 µM) or regorafenib (0.5 µM) for additional 40 h, and cell survival was measured by MTT assay. Data represent mean ± SEM  from four (d–f) independent experiments. ***P < 0.001, ###P < 0.001 compared with EV transfected. αααP < 0.001, βββP < 0.001 compared with TNFAIP8 transfected. EV: empty vector, NS: not significant, 3-MA: 3-methyladenine, Chlor: chloroquine, Sora: sorafenib, Rego: regorafenib.

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