Fig. 4: Prdm3 deletion in pancreatic acinar cells exaggerates cerulein-induced pancreatitis.
From: PRDM3 attenuates pancreatitis and pancreatic tumorigenesis by regulating inflammatory response
a Schematic illustration of experimental design. Ptf1aCreER;Prdm3flox/flox mice at 4 to 5 weeks of age were injected 4 times on alternating days with tamoxifen. One week after the last tamoxifen injection, mice were subjected to cerulein (50 μg/kg) injection at hourly intervals for 8 h per day for two consecutive days and analyzed at the indicated time points. b Histologic characterization was determined with hematoxylin-eosin staining (H&E). Immunohistochemistry staining for neutrophil marker Ly6B.2 and macrophage marker F4/80 with quantitation of the percent of all cells that are Ly6B.2+ or F4/80+ in Ptf1aCreER (control, n = 7) and Prdm3ΔAcinar (n = 5) pancreata. Vacuole is indicated by yellow arrowheads, and infiltrated immune cells are indicated by red arrowheads. c Serum amylase levels of cerulein-treated control (n = 11) vs. Prdm3ΔAcinar (n = 11) mice and saline-treated control (n = 3) vs. Prdm3ΔAcinar (n = 3). d Representative images of control and Prdm3ΔAcinar pancreata stained with H&E and the ductal marker Cytokeratin 19 (CK19). Quantification of the respective percent of all cells that are CK19+ from control (n = 6) vs. Prdm3ΔAcinar (n = 5) pancreata. Data show mean ± SD. Statistical analysis: Two-tailed t-test. *p < 0.05. Scale: 100 μm.
