Fig. 3: Impaired fatty acid oxidation (FAO) and cardiac lipid accumulation are caused by PHB2 ablation.
From: Prohibitin 2 deficiency impairs cardiac fatty acid oxidation and causes heart failure
a Representative confocal images of lipid droplets (LD) visualized by BODIPY™ 493/503 in isolated cardiomyocytes from 6-week-old WT and Phb2 cKO mice (left). Scale bar: 20 μm. The enlargement is the magnification of the boxed inset shown in left panel. Scale bar: 5 μm. Statistics of fold change of LD number, area and fluorescence intensity per cardiomyocyte (right). n = 80–100 cells from six mice per group. b Measurement of fatty acid oxidation (FAO) in isolated cardiomyocytes from 6-week-old WT and Phb2 cKO mice. Arrowhead indicates the time points of adding BSA (0.17 mM) or BSA-Pal (Palmitate) (1 mM). n = 3 mice per group. c Representative confocal images of fatty acid uptake (FAU) visualized by BODIPY™ 500/510 in isolated cardiomyocytes from 6-week-old WT and Phb2 cKO mice (top). Statistics of FAU levels (bottom). n = 80–100 cells from six mice per group. Scale bar: 50 μm. All Data represent mean ± SEM. Significance was determined by two-tailed, unpaired Student’s t test. ns: no significance. **p < 0.01, ***p < 0.001 versus WT group.
