Fig. 1: Pathway analysis of gentamicin-exposed human proximal tubule epithelial (HK-2) cells identified signal events in the Unfolded Protein Response (UPR) as potential contributors to injury.
From: Cross organelle stress response disruption promotes gentamicin-induced proteotoxicity
a HK-2 cells infected with an shRNA-based lentiviral signal gene library were exposed to gentamicin prior to RNAi analysis, and the scatterplot of shRNA abundance is shown. A threshold of ±2.5 STDEV was used to select significant changes in the abundance of signal-specific shRNA that either protected or sensitized renal cells to gentamicin. Red = increased abundance; Green = decreased abundance; Gray = unchanged vs. control shRNA. b Pathway analysis of significant shRNA changes yielded 226 genes within 11 distinct signal pathways that potentially mediate gentamicin-induced cell injury, including the UPR (shown in Supplementary Table 1). Blue nodes represent major signal pathways identified in our screen and orange nodes represent shared signal genes.
