Fig. 6: GGA reduces protein polyubiquitination and UPR activation in gentamicin-exposed renal cells.
From: Cross organelle stress response disruption promotes gentamicin-induced proteotoxicity
a Gentamicin increased protein degradation measured by polyubiquitination in cell lysates. b In gentamicin-exposed cells, GGA significantly reduced protein polyubiquitination to the baseline level; n = 7. c Immunoblot analysis shows BiP induction and downstream activation of all three UPR markers: ATF6, XBP1, and CHOP vs. beta-actin loading control. GGA decreased BiP, ATF6, and CHOP induction in gentamicin-exposed cells. Tunicamycin also increased steady-state BiP as well as the three UPR markers; GGA reduced all three UPR markers without preventing BiP induction; d GGA significantly reduced CHOP accumulation in gentamicin-exposed cells; Error bars = SEM; *P < 0.05; n = 6.
