Fig. 3: CD73-induced GC migration was regulated by c-Jun.
From: CD73 promotes tumor metastasis by modulating RICS/RhoA signaling and EMT in gastric cancer
a, c Western blot analysis of c-Jun-, CD73-, and EMT-associated proteins (E-cadherin, N-cadherin, vimentin, and β-catenin) after transfection of control vector or c-Jun for 48 h in MKN45-LV-NC or MKN45-CD73-RNAi cells and after treatment of SP600125 for 48 h in AGS-LV-CD73 cells. Relative gray value of indicated protein was shown (mean ± SD, n = 3, one-way ANOVA). b, d Transwell and wound healing assays were used to detect 3D and 2D migration capacity of MKN45 or AGS cells after treatment as indicated (mean ± SD, n = 5, one-way ANOVA). *p < 0.05, **p < 0.01, ***p < 0.001, and N.S., non-significant.
