Fig. 7: Genetic depletion of CD73 inhibits tumor metastasis of GC in vivo.
From: CD73 promotes tumor metastasis by modulating RICS/RhoA signaling and EMT in gastric cancer
a White-light images of peritoneal metastasis in the seeding metastasis model using MKN45 cancer cells (LV-NC, CD73-RNAi). Number of metastasis nodules and tumor volume were analyzed (mean ± SD, n = 5, unpaired t test). b White-light images of pulmonary metastasis in hematogenous metastasis model using MKN45 cancer cells and nodules were indicated with red arrow. The number of metastasis nodules and microinvasive area were analyzed (mean ± SD, n = 6, unpaired t test). c IHC staining of CD73 in metastasis nodules in seeding metastasis model and hematogenous metastasis model. d White-light images of gastric, intestinal, hepatic, and splenic tumor nodules and HE staining of metastasis nodules were shown. Red arrow indicates the nodules and red imaginary line indicates the border line of implanting sites (mean ± SD, n = 5, unpaired t test). e Diagram summarizing the role of CD73 in modulating metastasis process of GC. CD73-induced adenosinergic signaling modulated RICS function in the phosphorylation of RhoA-LIMK-cofilin signaling and activation of β-catenin-induced EMT process. *p < 0.05, **p < 0.01, and N.S., non-significant.
