Fig. 3: CR effect on density and diameter of Cx43 and Cx30 clusters. | Cell Death & Disease

Fig. 3: CR effect on density and diameter of Cx43 and Cx30 clusters.

From: Caloric restriction triggers morphofunctional remodeling of astrocytes and enhances synaptic plasticity in the mouse hippocampus

Fig. 3

a Triple immunocytochemical staining (GFAP, Cx30, and Cx43) of an astrocyte in hippocampal CA1 str. radiatum in control and CR mice. b Sholl-like analysis of connexin cluster distribution around the center of astrocyte soma. The number of clusters in each ring formed by neighboring circles was counted and divided by the ring area (cluster density). The distribution of cluster density with the distance from soma for Cx30 (c) and Cx43 (d). The distribution of mean cluster diameter with the distance from soma for Cx30 (e) and Cx43 (f). g Identification and interlinking of connexin clusters. The summary data are showing no changes in the mean distances between Cx30 clusters (h) and increased mean distances between Cx43 clusters (i) after CR. Gray diamonds—control, green diamonds—CR mice. The data are presented as mean ± SEM; NS. p > 0.05; *p < 0.05; two-sample t-test.

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