Fig. 6: CR reduces the activation of extrasynaptic NR2B-NMDA receptors. | Cell Death & Disease

Fig. 6: CR reduces the activation of extrasynaptic NR2B-NMDA receptors.

From: Caloric restriction triggers morphofunctional remodeling of astrocytes and enhances synaptic plasticity in the mouse hippocampus

Fig. 6

a Sample recordings of NMDARs-EPSCs in CA1 pyramidal neurons held at −20 mV in response to 5 × 50 Hz Schaffer collateral stimulation in the presence of AMPA and GABAA receptor blockers. Recordings were done at baseline condition (untreated, black trace), during application of NR2B-NMDAR antagonist Ro25-6981 (orange trace) and during subsequent application of broad-spectrum NMDAR antagonist APV (blue trace). Left—control, right—CR. Vertical dashed lines determine the regions of peaks and of the tail. b The time course of normalized charge transfer (area under the curve) of the area of the peaks of IPSCs. Gray squares—control, green circles—CR. c The effect of Ro25-6981 on the area under the NMDAR-EPSCs peaks. Gray diamonds—control, green diamonds—CR. d, e Same as (b), (c) but for the NMDARs-EPSCs tails. The data are presented as mean ± SEM; NS. p > 0.05; *p < 0.05; two-sample t-test.

Back to article page