Fig. 4: CLDN4 overexpression enhances HCC invasion and metastasis. | Cell Death & Disease

Fig. 4: CLDN4 overexpression enhances HCC invasion and metastasis.

From: Zinc finger protein 703 induces EMT and sorafenib resistance in hepatocellular carcinoma by transactivating CLDN4 expression

Fig. 4

A Upregulation of CLDN4 expression in SMMC7721-CLDN4 cell is assessed by Western blotting. B Transwell (B1) and wound-healing (B2) assay analysis of migration and invasion properties of SMMC7721-CLDN4 and SMMC7721-control cells. Scale bars: 250 μm (wound-healing) and 75 μm (transwell). C In vivo metastasis assays, (C1) Lung representative morphology and H&E staining, (C2) incidence of lung metastasis, (C3) number of metastatic lung foci, and (C4) overall survival of nude mice in SMMC7721-CLDN4 and SMMC7721-control groups (n = 10) are shown. Scale bars: 250 μm (low magnification) and 100 μm (high magnification). Data are presented asmean±S.D for three independent experiments. *P < 0.05.

Back to article page