Fig. 5: CLDN4 is essential for ZNF703-mediated HCC invasion and metastasis. | Cell Death & Disease

Fig. 5: CLDN4 is essential for ZNF703-mediated HCC invasion and metastasis.

From: Zinc finger protein 703 induces EMT and sorafenib resistance in hepatocellular carcinoma by transactivating CLDN4 expression

Fig. 5

A Western blotting analysis of ZNF703, CLDN4, E-cadherin, N-cadherin, Vimentin, and ZEB1 expression in upregulating or downregulating CLDN4 in the SMMC7721-ZNF703 or HCCLM3-shZNF703 cells, respectively. B Transwell (B1) and wound-healing (B2) assays demonstrate that upregulation of CLDN4 increases migration and invasion abilities of HCCLM3-shZNF703 cells, whereas downregulation of CLDN4 decreases migration and invasion abilities of SMMC7721-ZNF703 cells. Scale bars: 250 μm (wound-healing) and 75 μm (transwell). C In vivo metastasis assays, (C1) Lung representative morphology and H&E staining, (C2) incidence of lung metastasis, (C3) number of metastatic lung foci, and (C4) overall survival of nude mice in SMMC7721-ZNF703 cells downregulating CLDN4 and HCCLM3-shZNF703 cells upregulating CLDN4 groups (n = 10) are shown. Scale bars: 250 μm (low magnification) and 100 μm (high magnification). Data are presented as mean ± S.D for three independent experiments. *P < 0.05.

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