Fig. 1: hnRNP A0 inhibited the tumor cell progression and was abnormally expressed in colorectal cancer. | Cell Death & Disease

Fig. 1: hnRNP A0 inhibited the tumor cell progression and was abnormally expressed in colorectal cancer.

From: A tumor-specific modulation of heterogeneous ribonucleoprotein A0 promotes excessive mitosis and growth in colorectal cancer cells

Fig. 1

An SRB assay revealed that the numbers of hnRNP-knocked-down HCT116 cells, especially hnRNP A0-knockdown cells, were significantly lower than in the control (scramble) group a (n = 5). The growth of HCT116, MKN45, SUIT-2, PANC-1, and OE33 cells, but not CoEpiC, HCEC-1CT, and Het1A cells, was significantly reduced by the downregulation of hnRNP A0 b (n = 5). The cell growth of HCEC-1CT and Het1A cells, but not HCT116 cells, was significantly increased by the overexpression of hnRNP A0 c (n = 5). RT-PCR revealed that the overexpression of mRNA of HNRNP A0 was confirmed in a colorectal cancer cell line (HCT116 cells d; n = 3) and patients e (tumor: n = 29, normal: n = 21). A bioinformatic analysis using GEPIA (http://gepia.cancer-pku.cn/) revealed the overexpression of mRNA of HNRNP A0 in colorectal cancer patients f. In the xenograft model, the enlargement of the tumors in the HNRNP A0-knocked-down group was almost completely suppressed, while the tumors in the control (scramble) group became enlarged g (n = 5). Scramble, scramble siRNA. The error bars show the standard deviation (S.D.). *p < 0.05 by Student’s t-test.

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