Fig. 2: hnRNP A0-knocked-down colorectal cancer cells showed inhibition of mitotic events and induction of apoptosis. | Cell Death & Disease

Fig. 2: hnRNP A0-knocked-down colorectal cancer cells showed inhibition of mitotic events and induction of apoptosis.

From: A tumor-specific modulation of heterogeneous ribonucleoprotein A0 promotes excessive mitosis and growth in colorectal cancer cells

Fig. 2

A flow cytometry analysis revealed that HNRNP A0-knocked-down HCT116 cells accumulated in the G2/M phase of the cell cycle at 48 h a. Immunocytochemistry revealed abnormal spindle thread formation at 48 h. Scale bar: 10 µm b. Western blotting revealed that the expression of cleaved caspase-3 and PARP in HCT116 cells was increased by the downregulation of hnRNP A0 at 48 h c (n = 3). The number of TUNEL-positive HCT116 cells was increased by the downregulation of hnRNP A0 at 48 h. Scale bar: 100 µm d (n = 3). Scramble, scramble siRNA. The error bars show the S.D. *p < 0.05 by Student’s t-test.

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