Fig. 3: hnRNP A0 stabilized the mRNA of RAB3GAP1 and regulated the mitotic events in colorectal cancer cells.

hnRNP A0 was immunoprecipitated from the lysate of HCT116 cells. RNAs were extracted from the precipitant, and then a transcriptome analysis was performed to clarify the hnRNP A0 interacting mRNAs in HCT116 cells. The changes in mRNAs induced by hnRNP A0 downregulation were assessed using a transcriptome analysis of the siRNA of hnRNP A0-transfected HCT116 cells. The combination of immunoprecipitation and a transcriptome analysis revealed the 26 mRNAs that were directly bound to hnRNP A0 and stabilized by hnRNP A0 in HCT116 cells a (n = 3). An SRB assay showed that the cell growth was reduced by the downregulation of hnRNP A0-interacting mRNAs. The cell viabilities of HCT116 cells was <0.5 when mRNAs of NUDT-12, OPN3, and RAB3GAP1 were knocked-down b (n = 5). Western blotting revealed that cleaved caspase-3 and PARP were increased by the downregulation of these mRNAs as well as hnRNP A0 at 48 h c (n = 3). Flow cytometry showed that the cells accumulated in the G2/M phase of the cell cycle through the downregulation of hnRNP A0, RAB3GAP1, and OPN3 at 48 h d. Scramble, scramble siRNA. The error bars and numbers show the S.D. *p < 0.05 by Student’s t-test and an ANOVA.