Fig. 1: CircZNF609 is highly expressed and featured with loop structure in HCC. | Cell Death & Disease

Fig. 1: CircZNF609 is highly expressed and featured with loop structure in HCC.

From: CircZNF609 enhances hepatocellular carcinoma cell proliferation, metastasis, and stemness by activating the Hedgehog pathway through the regulation of miR-15a-5p/15b-5p and GLI2 expressions

Fig. 1

a RT-qPCR assay was conducted to detect the expression level of circZNF609 in HCC cell lines (HepG2, Huh-7, HCCLM3, and MHCC-97H) and normal human liver epithelial cell line (THLE-3). b The genomic location of circZNF609 together with its splicing pattern was displayed. c Nucleic acid electrophoresis analysis revealed that divergent primers amplified circular isoform of ZNF609 with cDNA, but not with genomic DNA (gDNA). GAPDH was used as a negative control. d The resistance of circZNF609 and ZNF609 mRNA to ActD was detected by RT-qPCR in HCCLM3 and MHCC-97H cells. e RT-qPCR assay was carried out to determine the abundance of circZNF609 and linear ZNF609 mRNA in HCCLM3 and MHCC-97H cells treated with RNase R (normalized to mock treatment). f The expression status of circZNF609 in HCC tissues and peri-tumor tissues was detected via RT-qPCR. *P < 0.05, **P < 0.01.

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